Kupulikiska Mikhaliro Yikuru Na Malango Ghakupatulira Maselo gha Biocoll

BioColl maselo kupatukana yankho ndi m'badwo uphya wa kupatukana yankho kukhazikiska na Biochrom, Germany. Padera pa kupatura maselo gha lymphocytes, ŵakugwiliskira nchito comene pakupatura maselo gha pa cirwa. Kuŵika maselo gha pa chilumba ni nthowa yiphya yakuchizgira matenda gha shuga, iyo yingamazga kusoŵa kwa maselo gha pa chilumba na kugwira ntchito mwakukwana yayi, chomenechomene ku ŵalwari awo ŵali na suzgo la shuga la mtundu I.

Vigaŵa:

1. Kupereka maselo ghakupambanapambana gha Biocoll ghakupatulika, agho ghangapatura maselo gha mononuclear kufuma ku ndopa zakuzungulira, mnofu wa viwangwa na ndopa za chingwe cha umbilical, maselo gha stromal ghakucepa-, maselo gha mesenchymal stem, na maselo gha ndopa gha rodent.

2, ndondomeko ya kupanga vinthu mwakuyana na GMP na United States Pharmacopoeia, vyambura kubaba ndiposo vyakunozgeka kugwiliskira nchito, vyakuchepa vya endotoxin.

3. The separation effect is good, the obtained cell activity >80%, FICOLL yingaŵa yakujiyimira payokha, ndipo nyengo yakugwilira ntchito njitali.

Tikwenera kuŵikapo mtima pa nkhani izi .

Nthowa yakuŵikira vinthu paŵekha yingakhwaska vipambi na umoyo wa vilumba vichokovichoko, ntheura nchakukhumbikwa kuŵa maso para mukutora viyezgelero .

1. Chiwindi chikwenera kutoleka mwaluŵiro ndipo kapusulu yikwenera kusungika makora.

2. Ntchiwemi kugwiliskira ntchito yankho la UW pa vyakwendekwenda.

3. Chiwindi chikayezgeka kuti chiwuskemo mafuta ghanandi na duodenum yakukhalira na spleen pambere ŵandaŵikemo enzyme yakugaya vyakurya.

4. Nthowa yakugaŵira vyakurya yikwenera yayi kutimbanizgika kuti viwaro vya chiwindi vigayikenge na maji ghakugaya vyakurya agho ghakuŵikika m’malo mwa trypsin iyo yikufuma mu chiwindi.

5. Kuti muwoneseske kuti envelopu ya chilumba njakugomezgeka, kugaya vyakurya kungaŵa kwakuzura chomene yayi. Ntchiwemi kuchita vichoko chomene kuluska kuchita vinandi chomene, usange yayi, vipambi vya kutozga chirwa chizamukhwaskika.

1. Sakanizgani 7mL na 10mL mwakuyana

Biocoll maselo ghakupatulika (D =1.077 g / mL, 20 digiri ) ghakaŵikika mu 15mL na 25mL machubu ghakuŵikamo.

2. Sakanizgani ndopa zose za heparinized anticoagulant (50U / mL heparin, palije stabilizer) na ndopa zakukwana nyengo zose mwakuyana waka, ndipo ziŵikeni mwakupwelelera pa maji ghakupangika na maji ghakupatura.

3. Kuŵika pa 1200g kwa mphindi 20. Layer yakukhozga maselo gha thupi (iyo yili pakati pa plasma na Biocoll) yikakoleka na pipet ya Pasteur (70-100%), yikasambizgika kaŵiri mu chigaŵa chapakati, yikaŵikika mu * 300g kwa maminiti 10, ndipo pa * 200g kwa maminiti 10. Ŵakasanga maselo gha lymphocytes agho ghakakhumbikwiranga, ndipo ŵakapendanga maselo na kuyezga vinthu vinyake mwakuyana na nthowa zakumanyikwa.