Anti-DE Pseudorabies Virus Antibody Kit

Phafififize

PRV{{0}gE Ab Elisa

Kugwiliskira ntchito chomene:Nthowa za Porcine Virus gE Kujara

Antibody ELISA Dection Kit ndiyo yikusankha gE antibody mu seramu ya nkhumba ya virus ya nkhumba ya nkhumba mu vitro. Ŵanthu ŵakugwiliskira ntchito mayeso agha kuti ŵapambaniske mu thengere .

virus na katemera kufuma ku katemera wa gE gene deletion katemera.

Vinthu: seramu

Vinthu Vikukhumbikwa Kweni Kupeleka yayi:

Maji ghakutowa

munthu munyakhe

96-mall Microplate wakuŵazga (450nm)

Tiyini

Centrifuge (4000rpm)

Makina ghakugeza

Oveni (37℃.

 

Nthowa zakuyezga & Kulongosora

STEP 1 Nthowa Yakuyezga

1.1 Kunozga Nthowa za Wash

Wkuŵika kuŵika maghanoghano ghake pa wphulusa:Pambere mundagwiriskike nchito, nthowa yakuchapira yikwenera kuwezgeka ku kutentha kwa chipinda (20-25℃) na kusunkhunyika mwakuyana kuti yiŵe yakuyana waka. Pamanyuma pake, dilute 20 nyengo zakuchapira na maji ghakutozgeka (mwaciyelezgero, nthowa yakuchapira ya 5ml yikusazgikira ku maji ghakutozga gha 95ml), kusakanizga makora, ndipo maji ghakugezeramo ghakudumulika ghangasungika pa 2-8℃kwa mazuŵa 7.

1.2 Chiyelezgero

Nyama yiliyose yikatoreka, centrifuged pa 4000rpm kwa maminiti 10, ndipo supernatant yikasonkheka. Seramu nayo wangaŵa wakuchimbira mwachibadwa kwambura hemolysis.

② Delute sampulo ya seramu 1:1 (kuwonjezera 70ul seramu sampulo ku 70ul sampulo ya diluent) na kusakanizga makora.

Ziŵani kuti: Vinthu ivyo vikupangiska kuti munthu waŵe na suzgo la maghanoghano vikwenera yayi kuchepeskeka.

1.3 Nthowa

Pangani chiziŵizgo cha enzyme Microplate (kuyana na chiŵelengero cha masampulo, chingagaŵika na kugwiliskirika ntchito kanandi waka), kusazgapo 100ul ya seramu yakuchepeskeka ku enzyme chikwangwani cha Microplate, kuŵika visimi viŵiri pa chilichose kuti viŵe viheni na viwemi, ndipo vikusazgapo 100ul chilichose pa vigaŵa viheni na viwemi ku vigaŵa vyakuchitira vinthu mwakuyana. Kusunkhunya mwakuzika sampulo mu chisimi, kugwiriskira ncito filimu ya mbale yakudinda kuti yibenekelere mbale, na kuŵika pa 37℃kwa maminiti 60.

‐Jepen filimu ya chidindo cha chidindo, kugwedezga yankho mu chisimi cha mbale, ŵikanipo 300μl ya maji ghakuchapiramo ghakugwira ntchito ku chisimi chilichose, ndipo pamanyuma mutaye maji mu chisimi. Bwerezganipo masitepe agho ghali pacanya apa maulendo 5, ndipo paumaliro muwume pa pepara lakutowa lakupokera maji.

‐Add 100μl ya Enzyme Conjugate ku chisimi chilichose, kuphimba mbale, na kuŵika pa 37℃kwa maminiti 30.

‐Open filimu ya chidindo cha chidindo, sunkhunyani nthowa iyo yili mu chisimi cha mbale, ŵikanipo 300μl ya maji ghakuchapira ghakugwira ntchito ku chisimi chilichose, ndipo pamanyuma mutaye maji mu chisimi. Bwerezganipo masitepe agho ghali pacanya apa maulendo 5, ndipo paumaliro muwume pa pepara lakutowa lakupokera maji.

‐Add 100μl substrate yankho ku chisimi chilichose, kuphimba mbale na kuŵika kwa maminiti 15 pa 37℃kutali na ungweru.

‥Addd 50 μl stop trafer ku chisimi chilichose, na kusankha ivyo vikuchitika mu mphindi 5.

STEP 2 Kulongosoraso

Mtengo wa OD450 wa chisimi chilichose ukaŵazgika pa 96-well microplate wakuŵazga.

2.1 Kuwonekera

The average OD450nm value of negative control was ≥ 0.5 and the blocking rate of positive control was >50%, vyakuchitika vyakuyezgayezga vikaŵa vyakwenelera. Kupatula apo, mayeso ghakwenera kuwerezgeka.

2.2 Nthowa yakuŵerengera

Pa sampulo yiliyose, kupenda PI (Blockingr ate) nga ni umo vikuŵira pakugwiriskira ncito sampulo yakunozgeka na vigaŵa vyakulongozga:

2.3 Vinthu ivyo vikupangiska kuti ŵanthu ŵaŵe na mwaŵi wa kuŵika vinthu pa malo ghanyake.

Para PI < 40%, yikuwoneka kuti njambura kwenelera.

Para PI > 50%, yikuwoneka kuti njakukhozga.

Para 40% Kuchepa panji kufanana na PI Less kuluska panji kufanana na 50%, kukuyeruzgika kuti nkhwakukayikiska, ndipo kukupereka kuwerezgapo kubwerezga na kuyezga sabata yimoza pamanyuma pake.

Tag yakutentha: anti{0}} } poscine pseudorabies mavairasi antibody elisa kit, China anti- ge pseudorabies pseudorabies mavairasi mavairasi antibody elisa elisa kit, ŵakupanga, fakitare .